The GSL core facility in Stanley Hall is equipped with two HiSeq 2000 DNA sequencers from Illumina. A third HiSeq 2000 is located in the Functional Genomics Laboratory (FGL) in LSA. Our mission is to make massively paralleled DNA sequencing affordable to UC Berkeley, the greater UC community and outside clients. Applications include whole genome sequencing, genomic resequencing, ChIP-sequencing, RNA expression, small RNA discovery and bisulfite sequencing.

The GSL accepts sample drop-offs in Stanley Hall B206 from 10 a.m.-6 p.m., Mon.-Fri. Sequencing library samples can also be dropped off in the LSA site (255 LSA) from 9 a.m.-5 p.m., Mon.-Fri.

With the changeover to the HiSeqs and the increased barcoding or indexing of samples, the qPCR quality control step has become more of a bottleneck in the workflow than actual sequencer run time. We are therefore asking that labs that have access to qPCR machines use the Kapa Biosystems kits to perform qPCR quality control PCR reactions on their own library samples. Profiles of these qPCR reactions should be turned in along with their samples and Agilent Bioanalyzer traces.

For assistance or information, please contact Minyong Chung, GSL Staff Research Associate II, Minyongis@berkeley.edu, 510-666-3372.

You may also contact Justin Choi, jygchoi@berkeley.edu, and Karen Lundy, lundyk@berkeley.edu, in the Functional Genomics Laboratory, 510-642-1165.