The Illumina HiScan instrument utilizes Illumina’s BeadArray Technology which is based on 3-micron silica beads that self assemble in microwells on either of two substrates: fiber optic bundles or planar silica slides. When randomly assembled on one of these two substrates, the beads have a uniform spacing of ~5.7 microns. Each bead is covered with hundreds of thousands of copies of a specific oligonucleotide that act as the capture sequences in one of Illumina’s assays.
The HiScan System provides the fastest scan times for Illumina’s high-multiplex BeadChips. The HiScan grants users access to a range of applications, including whole-genome genotyping, gene expression analysis, methylation analysis, and more. The following are currently provided at the GSL. Unfortunately, we are no longer supporting the GoldenGate assays.
The GSL provides the following Assays on the HiScan:
Genotyping Platforms Currently Offered:
Illumina’s Infinium Genotyping Assay
Illumina’s Infinium HD BeadChips profile the human genome with 300,000 to approx. 1.2 million markers. With Illumina’s Omni family of microarrays, there’s a wide selection of flexible arrays featuring markers selected from the International HapMap and 1000 Genomes Projects.
Examples of Infinium BeadChips that we process: HumanOmniExpress, HumanExome, Human Immuno DNA Analysis BeadChip, Human Omni1-quad, and Human Omni1S
Illumina’s Infinium HumanMethylation450 BeadChip allows the interrogation of >485,000 methylation sites per sample. The content covers 99% of RefSeq genes and 96% of CpG islands.
Other assays the GSL plans to support:
Whole-Genome Expression Beadchips for Gene Expression Analysis (specifically the HumanHT-12 Expression BeadChip)
Omni Arrays: HumanOmni2.5-8, HumanOmni2.5S, and HumanOmni5-Quad.
Due to the customizable nature and larger scope of these projects, pricing is provided on a project basis. Please contact us with your project needs and we will provide you with a quote inclusive of all QC, sample handling, assay consumables, preliminary data analysis and data delivery.
If you have any questions regarding our Illumina Array-Based Services on the HiScan or would like to get started, please contact:
Hong Quach (Illumina Arrays and Biospecimen Processing)
431 Stanley Hall
Berkeley, CA 94720-3220