Our purified Cas9-NLS protein is available to all users.
We express and purify the S. pyogenes Cas9 protein with a double nuclear-localization tag at the C-terminus. This is formulated at 40 uM in 20 mM HEPES-KOH pH 7.5, 150 mM KCl, 10% glycerol, 1 mM DTT.
Cost per 2.5 mg unit (40 tubes) is $250 for UC users (non-UC users please contact us for a quote).
We can purify a variety of other Cas9 variants on request. Contact us for details.