We are well-seasoned gene jockeys, just ask and chances are we can clone it.
We routinely take on small (<10 samples) and large (48 or 96 samples) scale projects. Please see our price sheet to get an idea of our rates. Don’t hesitate to contact us with any questions.
We routinely use LIC (ligation independent cloning) and SLIC (sequence and ligation independent cloning) to make our plasmids. We can clone your gene into your plasmid or one of the many bacterial or bacculovirus expression plasmids we have available. We also can synthsize custom vectors to provide the exact sequence you desire.
Click here to download our expression vector lists.
(Includes: a summary of all our plasmids, full plasmid sequences, and our cloning protocols)
All of the MacroLab Vectors are available at Addgene.org. For UC Berkeley labs, contact us directly.
If you are interested in obtaining our entire collection (as of DEC 2013) of 135 cloning plasmids please contact Scott Gradia. The two-plate set of glycerol stocks is available to UC labs for $900 and non-UC labs for $1400.
If we don’t have a vector that suits your need, it is likely that we can use SLIC or traditional restriction enzymes to clone into any vector you want.
For more information about LIC cloning, follow the link below:
Some helpful files are available for you to download below:
Easily design primers with LIC compatible ends using the following spreadsheet. Just enter the open reading frame that you want to amplify.
Use the following spreadsheet to obtain the amino acid sequence, molecular weight, extinction coefficient, and PCR primers from your DNA sequence.
Please ask for guidence before you sit down and design your 96 well project. Hopefully we can save you a lot of time and effort.