The open access MALDI instrument is located in B208B Stanley Hall and is available to authorized users 24 hours a day, seven days a week. Authorized users may book time using a Google calendar (access will be provided when you sign up to use the MALDI instrument).
Groups that are interested in using the MALDI mass spectrometer should appoint a group operator. Group operators are expected to train other members of their group who wish to use the instrument. Graduate students who are assigned the responsibility of being a group operator are expected to complete CHEM268 as part of their training or have equivalent prior experience. CHEM268 (Chemical and Biological Mass Spectrometry) is offered in the spring semester each year. Exceptions to this requirement will be made on a case-by-case basis by the facility manager. For more information about the open access MALDI instrument, training, and group operator responsibilities, please contact Dr. Ulla Andersen (email: norklit AT berkeley DOT edu).
Voyager DE PRO
The MALDI instrument is a Voyager DE Pro from Applied Biosystems. It operates both in linear and reflector mode.
New sample plates can be obtained from JBI Scientific. The most common plates is a stainless steel plate with room for 100 samples. The catalogue number is V700666, the list price should be around $250.
In matrix-assisted laser desorption ionization (MALDI), the sample is embedded in a low molecular weight, UV-absorbing matrix that enhances desorption and ionization of the analyte. Sample preparation is crucial for successful MALDI. It is important to choose the correct matrix for your sample and to use suitable concentrations of both the sample and the matrix.
Commonly used MALDI matrices:
a-cyano-4-hydroxy-cinnamic acid (CHCA)
peptides, small proteins (<10 kDa)
Proteins (>10 kDa)
2,5-dihydroxy benzoic acid (DHB)
peptides, small proteins, small molecules, neutral carbohydrates, synthetic polymers, oligonucleotides (<10 bases)
2,4,6 trihydroxy acetophenone (THAP)
small oligonucleotides, acid sensitive compounds
Tips on how to spot your sample can be found here: Spotting tech.ppt
Tips on how to remove buffers, salts, urea, guanidine, EDTA, glycerol, DMSO and detergents from your MALDI spots can be found here: MALDI Clean-up.ppt
Groups that are interested in using the MALDI instrument should appoint a group operator. Each group is responsible for supplying their own sample plates, matrices and other supplies necessary for sample preparation (micropipettes, micro centrifuge tubes and solvents).