Enzymatic digestion of proteins and protein mixtures
Use this protocol to convert your protein samples to peptides in preparation for mass spectral analysis.
Use this protocol to desalt peptides prepared by enzymatic digestion in solution. It is not necessary to desalt peptide samples prepared from gel bands.
Enzymatic digestion of proteins from gel bands
Use this protocol for preparation of samples from SDS gel bands.
TCA precipitation of proteins
This protocol is an optional cleanup step prior to enzymatic digestion. It can be used to concentrate samples and to remove some non-protein contaminants.
Use this protocol to prepare samples labeled with isobaric tags for relative quantitation.
Please read our sample preparation tips if you are new to these protocols.