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UC Berkeley: Biomolecular Nanotechnology Center
Biomolecular Nanotechnology Center Services: Lithography microfabrication, soft lithography and glass or polymer bonding capabilities; a full range of deposition, etching, metrology, and microscopy equipment. Available instrumentation includes NanoSurf Atomic Force Microscope, FEI Quanta 3D FEG FIB, FEI SCIOS 2 LoVac Dual Beam FIB, Zeiss Orion NanoFab, and Keyence 3D laser profile measurement system.
Manager: Paul Lum
UC Berkeley: Cal-Cryo@QB3
Cal-Cryo@QB3 Services: Instrumentation for cryoEM, including:
Krios G2 300kV CryoTEM with GATAN K3 Direct Electron Detector and Volta Phase Plate; BIO Quantum Energy Filter; Krios G3i 300kV CryoTEM with GATAN K3 Direct Electron Detector and BIO Quantum Energy Filter; Talos Arctica 200kV CryoTEM with Gatan K3 Direct Electron Detector; and FEI Mark IV Vitrobot.
Manager: Daniel Toso, Director
UC Berkeley: Cell and Tissue Analysis Facility
Cell and Tissue Analysis Facility Services: Cell culture equipment and high-end instrumentation for mammalian cells, tissues, proteins, and more from flow cytometry to multiplex ELISA to automated epifluorescence, confocal, and multiphoton imaging.
Instruments include: Biospherix hypoxia chambers; ImageXpress Micro, Molecular Devices; Lionheart FX, Agilent/Biotek; Multiphoton photon microscopy Bruker/Prairie Technologies; Attune NxT acoustic focusing cytometer; histology suite with Cryostar NX50 cryostat, freezing stage sliding microtome, and paraffin embedding and sectioning.
Manager: Mary West
UC Berkeley: High-Throughput Screening Center
High-Throughput Screening Center Services:
Provides access to cell culturing space, automated liquid handling, automated plate reader and high-throughput, high-content microscopy instrumentation for screening experiments, along with tools for analysis and data visualization. Emphasis is on whole genome and sub-library siRNA screening from various sources but other high-throughput fluidics projects are welcome.
Instruments include:
V11 Bravo Automated Liquid Handler, Agilent with Inline Plate Washer and Sealer; BioMek NX with Span-8, Beckman-Coulter; Muitiflo FX MultiMode Dispenser, Agilent/Biotek; EVOS FL Color Imaging System, ThermoFisher; Envision MultiMode Plate Reader, Perkin-Elmer; Automated Confocal/Wide-field/Transmitted Light Microscope Opera Phenix, and Perkin Elmer.
Manager: Mary West
UC Berkeley: Pines Magnetic Resonance Center@QB3
Pines Magnetic Resonance Center@QB3 Services: The Pines Magnetic Resonance Center comprises a groundbreaking innovation and development center in NMR, outreach and education, and an analytical facility with leading-edge, advanced instrumentation, including the NMR facility in Stanley Hall, now the Pines Magnetic Resonance Center @QB3 (PMRC@QB3). The PMRC@QB3 is administered by the Pines Magnetic Resonance Center – Core Facility.
Manager: Hasan Celik
UC Berkeley: QB3/Chemistry Mass Spectrometry Facility
QB3/Chemistry Mass Spectrometry Facility Services: Provides routine acquisition of mass spectra and accurate mass measurements of biological, organic, and inorganic compounds, as well as mass measurements of intact proteins, lipids, oligosaccharides, and non-covalent protein-protein and protein-ligand complexes and supramolecular coordination clusters. The facility also provides advanced proteomics analysis and operates an open-access laboratory. The facility also operates an open access MALDI-TOF instrument available to trained users.
Manager: Ulla Andersen
UC Berkeley: QB3 Genomics
QB3 Genomics Services: Provides QC and sequencing services. Instrumentation includes Illumina MiSeq, NextSeq and NovaSeq platforms, as well as a PacBio Sequel II system and Oxford Nanopore Minion system. PacBio and ONT library preps and high molecular weight extractions. Library preparation, QC, purification, enrichment, and single cell (10X) services.
Manager: Shana McDevitt
UC Berkeley: QB3 MacroLab
QB3 MacroLab Services: Offers gene cloning, protein expression and protein purification services. Also provides reagents such as competent cells, Cas9 proteins, and TEV protease.
Manager: Chris Jeans
UC Berkeley: Vincent J. Coates Proteomics/Mass Spectrometry Facility
Vincent J. Coates Proteomics/Mass Spectrometry Facility services: Provides comprehensive proteomics services using mass spectrometry, determining the protein contents of samples as simple as gel bands or as complicated as whole cell extracts. The facility provides identification of post-translational modifications and relative quantitation as well as consultation regarding sample preparation and experimental design.
The facility provides proteomics services principally through mass spectrometry. Currently the facility has two Thermo LTQ XL linear ion trap mass spectrometers and a Thermo Fusion Lumos mass spectrometer.
Manager: Robert Maxwell
