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UC Berkeley: Biomolecular Nanotechnology Center
Biomolecular Nanotechnology Center Services: Lithography microfabrication, soft lithography and glass or polymer bonding capabilities; a full range of deposition, etching, metrology, and microscopy equipment. Available instrumentation includes NanoSurf Atomic Force Microscope, FEI Quanta 3D FEG FIB, FEI SCIOS 2 LoVac Dual Beam FIB, Zeiss Orion NanoFab, and Keyence 3D laser profile measurement system.
Manager: Paul Lum
UC Berkeley: Cal-Cryo@QB3
Cal-Cryo@QB3 Services: Instrumentation for cryoEM, including:
Krios G2 300kV CryoTEM with GATAN K3 Direct Electron Detector and Volta Phase Plate; BIO Quantum Energy Filter; Krios G3i 300kV CryoTEM with GATAN K3 Direct Electron Detector and BIO Quantum Energy Filter; Talos Arctica 200kV CryoTEM with Gatan K3 Direct Electron Detector; and FEI Mark IV Vitrobot.
Scientific Director: Kedar Sharma
UC Berkeley: Cell and Tissue Analysis Facility
Cell and Tissue Analysis Facility Services: Cell culture equipment and high-end instrumentation for mammalian cells, tissues, proteins, and more from flow cytometry to multiplex ELISA to automated epifluorescence, confocal, and multiphoton imaging.
Instruments include: Biospherix hypoxia chambers; ImageXpress Micro, Molecular Devices; Lionheart FX, Agilent/Biotek; Multiphoton photon microscopy Bruker/Prairie Technologies; Attune NxT acoustic focusing cytometer; histology suite with Cryostar NX50 cryostat, freezing stage sliding microtome, and paraffin embedding and sectioning.
Manager: Mary West
UC Berkeley: High-Throughput Screening Center
High-Throughput Screening Center Services:
Provides access to cell culturing space, automated liquid handling, automated plate reader and high-throughput, high-content microscopy instrumentation for screening experiments, along with tools for analysis and data visualization. Emphasis is on whole genome and sub-library siRNA screening from various sources but other high-throughput fluidics projects are welcome.
Instruments include:
V11 Bravo Automated Liquid Handler, Agilent with Inline Plate Washer and Sealer; BioMek NX with Span-8, Beckman-Coulter; Muitiflo FX MultiMode Dispenser, Agilent/Biotek; EVOS FL Color Imaging System, ThermoFisher; Envision MultiMode Plate Reader, Perkin-Elmer; Automated Confocal/Wide-field/Transmitted Light Microscope Opera Phenix, and Perkin Elmer.
Manager: Mary West
UC Berkeley: Pines Magnetic Resonance Center@QB3
Pines Magnetic Resonance Center@QB3 Services: The Pines Magnetic Resonance Center comprises a groundbreaking innovation and development center in NMR, outreach and education, and an analytical facility with leading-edge, advanced instrumentation, including the NMR facility in Stanley Hall, now the Pines Magnetic Resonance Center @QB3 (PMRC@QB3). The PMRC@QB3 is administered by the Pines Magnetic Resonance Center – Core Facility.
Manager: Hasan Celik
UC Berkeley: QB3 Mass Spectrometry
QB3 Mass Spectrometry’s mission is to provide UC Berkeley and the broader scientific community with affordable access to cutting-edge mass spectrometry. The facilities specialize in the analysis of diverse species, ranging from small molecules to complex supramolecular clusters.
Managers:
Anthony Iavarone, general mass spectrometry
Rob Maxwell, proteomics
UC Berkeley: QB3 Genomics
QB3 Genomics Services: Provides QC and sequencing services. Instrumentation includes Illumina MiSeq, NextSeq and NovaSeq platforms, as well as a PacBio Sequel II system and Oxford Nanopore Minion system. PacBio and ONT library preps and high molecular weight extractions. Library preparation, QC, purification, enrichment, and single cell (10X) services.
Manager: Shana McDevitt
UC Berkeley: QB3 MacroLab
QB3 MacroLab Services: Offers gene cloning, protein expression and protein purification services. Also provides reagents such as competent cells, Cas9 proteins, and TEV protease.
Manager: Chris Jeans
