QB3-Berkeley offers shared research facilities to provide advanced instrumentation, technologies, and materials, along with the technical leaders to manage them and provide expert service. Access to these resources enables scientists and engineers to develop medical devices, drugs, and therapies that improve lives, as well as technologies to alleviate environmental damage and improve energy production and use.
These facilities are primarily used by UC-affiliated researchers, but may provide services in some cases to users from other academic institutions as well as industry and government labs. Please visit individual web sites to learn more about each facility’s capabilities and rates. To explore QB3 research facilities located at UC San Francisco and UC Santa Cruz, visit here.
BIOCHEMISTRY AND STRUCTURAL BIOLOGY
Cal-Cryo@QB3-Berkeley provides researchers with access to state-of-the-art cryo-electron microscopes and related tools for high resolution structural biology imaging experiments.
Central California 900 MHz NMR Facility
This facility promotes the understanding of the structures and dynamics of natural products, potential therapeutics, proteins, and nucleic acids at the atomic level. Instruments include a 900 MHz spectrometer equipped with a cryoprobe, along with several other NMR systems.
The QB3 MacroLab offers automated gene cloning, protein expression, protein purification, and crystallography, which enable researchers to expand the scope of protein-structure discovery and advance fundamental understanding of biochemistry.
CELLULAR/MATERIALS ANALYSIS AND MICROFABRICATION
Biomolecular Nanotechnology Center
The BNC is a fabrication and experimentation facility specializing in BioMEMS and microfluidic devices, offering lithography, deposition, etching, metrology, and microscopy equipment and facilities for biological experimentation. The BNC also houses instructional labs and is adjacent to QB3 incubator space.
QB3 Cell and Tissue Analysis Facility / High-Throughput Screening Facility
The QB3 Cell and Tissue Analysis Facility provides cell culture equipment and instrumentation needed to grow and assess stem cells from flow cytometry to multiplex ELISA reader to automated epifluorescence, confocal, and multiphoton imaging. The High-Throughput Screening Facility provides cell culturing space for automated cell seeding, and automated liquid handling equipment, multi-label plate reader and high-content imaging for any type of multi-well plate experiments.
Computational Genomics Resource Laboratory (CGRL)
For researchers who use next-generation genomic sequencing, the CGRL provides computational infrastructure, technical expertise, workshops, and training in experimental design and data analysis. Staff provide free consultation and analyses for NGS data. John Taylor and Brian Staskawicz direct this core facility.
Functional Genomics Laboratory
The FGL conducts research in functional genomics, specializing in single-cell genomics and the preparation of Next-Generation Sequencing (NGS) libraries. The facility also has available various user-accessible instruments to aid users in creating their own libraries. The FGL also specializes in the fabrication, use, and analysis of DNA microarrays for large-scale gene expression profiling and genetic profiling, and provides full services for Affymetrix GeneChip arrays and Agilent DNA microarrays. John Ngai is the faculty adviser for this facility.
Vincent J. Coates Genomics Sequencing Laboratory
The GSL provides high throughput DNA sequencing on Illumina’s HiSeq4000/2500 and MiSeq platforms, Illumina Array Services on the HiScan, and Biospecimen processing. Additional services include Illumina or PacBio project design consultation with library preparation facilitated through the FGL, and quality control services including the Qubit, Bioanalyzer, and qPCR assays. Don Rio is the faculty adviser for this facility.
QB3/Chemistry Mass Spectrometry Facility
The facility provides routine acquisition of mass spectra and accurate mass measurements of biological, organic, and inorganic compounds, as well as mass measurements of intact proteins, lipids, oligosaccharides, and non-covalent protein-protein and protein-ligand complexes and supramolecular coordination clusters. The facility also provides advanced proteomics analysis as well as operates the only ion mobility mass spectrometer on the UC Berkeley campus.
Vincent J. Coates Proteomics/Mass Spectrometry Laboratory
The proteomics / mass spectrometry laboratory (P/MSL) offers full services in protein identification and characterization. These services include protein identification from complex samples through MudPIT analysis, post-translational modification analysis, identification of proteins from gel bands, and relative quantitation.