The QB3 MacroLab is a core facility administered by QB3-Berkeley. Located in UC Berkeley’s Stanley Hall and directed by Professor Susan Marqusee, the QB3 MacroLab offers automated gene cloning and recombinant protein expression and purification services.
Academic and nonacademic researchers are eligible to purchase services from the QB3 MacroLab. To learn more about our services and how you can work with the facility, please contact facility manager Chris Jeans.
| MacroLab Rates for FY 22-23 (updated June 28, 2022) | UC Rate * | External Rate | QB3 startup partner rate | ||
| Competent cells (96 tubes; see our website for available strains) | $110.00 | $250.00 | $180.00 | ||
| LIC Cloning (each, up to 24 samples) | $210.00 | $528.00 | $369.00 | ||
| Single protein expression testing/optimization (E. coli only) | $345.00 | $882.00 | $613.50 | ||
| TEV protease (40 mg) | $315.00 | $885.00 | $600.00 | ||
| Cas9-NLS purified protein (2.5 mg) | $99.00 | $250.00 | $174.50 | ||
| Protein purification (2 L E. coli) | $800.00 | $1,900.00 | $1,350.00 | ||
| Custom work (hourly charge) | $66.00 | $153.00 | $109.50 | ||
| Phusion polymerase (500 μl, for 1000 reactions) | $58.00 | $154.00 | $106.00 | ||
| Cloning master mixes: Gibson (without ligase), LIC, SLIC (100 tubes per box) | $70.00 | $135.00 | $102.50 | ||
| Macrolab plasmids (10 μl aliquot). See our website for list of available plasmids | $11.00 | $88.00 | $49.50 | ||
| Shipping (dry ice) | $52.00 | $100.00 | $76.00 |
*UC Affiliates (such as LBNL) add 9.9% to the UC rate.
Contact
| Chris Jeans, Ph.D. Facility Director | c.jeans@berkeley.edu (email preferred) |
| Professor Susan Marqusee Faculty Director | marqusee@berkeley.edu |