The QB3 MacroLab is a core facility administered by the California Institute for Quantitative Biosciences (QB3). Located in UC Berkeley’s Stanley Hall and directed by Professor Susan Marqusee, the QB3 MacroLab offers an automated gene cloning and recombinant protein expression and purification services.
Academic and nonacademic researchers are eligible to purchase services from the QB3 MacroLab. To learn more about our services and how you can work with the facility, please contact facility manager Chris Jeans.
| MacroLab Project Costs (updated August 10, 2020) | UC Rate * |
| LIC Cloning (96-well format) | $1,885.00 |
| LIC Cloning (each, up to 24 samples) | $198.00 |
| E. coli protein expression testing (96-well format) | $1,000.00 |
| Protein purification (2 L E. coli) | $728.00 |
| Cas9-NLS purified protein (2.5 mg) | $90.00 |
| TEV protease (40 mg) | $306.00 |
| Phusion polymerase (500 μl, for 1000 reactions) | $50.00 |
| Competent cells (96 tubes; see our website for available strains) | $100.00 |
| Cloning master mixes: Gibson (without ligase), LIC, SLIC (100 tubes per box) | $64.00 |
| Macrolab plasmids (10 μl aliquot). See our website for list of available plasmids | $10.00 |
| Single protein expression testing/optimization (E. coli only) | $301.00 |
| Shipping (dry ice) | $52.00 |
| Custom work (hourly charge) | $60.00 |
| * UC Affiliates (such as LBNL) add 9.9% to the UC rate. | |
| Most services also available to non-UC labs. Please contact us for a quote. | |
Contact
| Chris Jeans, Ph.D. Facility Director | c.jeans@berkeley.edu (email preferred) |
| Professor Susan Marqusee Faculty Director | marqusee@berkeley.edu |