Cal-Cryo at QB3-Berkeley provides researchers with access to state-of-the-art Cryo Electron Microscopes and related tools for high resolution structural biology imaging experiments.
Meet Cal-Cryo at QB3-Berkeley
The Cal-Cryo facility, housed in the basement of Stanley Hall, is led by Professors James Hurley and Steve Brohawn and began operations in February 2018. Our facility is used to collect image data of biological or soft material samples before it is processed using single particle or tomographic reconstruction methods. Our staff offer consultation and training in specimen preparation, microscope operation, image acquisition, and data analysis.
The facility’s instrumentation features automated data collection. Both single-particle micrographs and tomography tilt series can be collected.
The current schedule can be viewed on this calendar.
Procedures and documentation for use of Cal-Cryo @QB3-Berkeley’s equipment are accessible in this folder.
Cal-Cryo@QB3-Berkeley instruments include:
- Krios G2 300kV CryoTEM
GATAN K3 Direct Electron Detector
Volta Phase Plate, BIO Quantum Energy Filter
- Krios G3i 300kV CryoTEM
GATAN K3 Direct Electron Detector
BIO Quantum Energy Filter
- Talos Arctica 200kV CryoTEM
Gatan K3 Direct Electron Detector
- FEI Mark IV Vitrobot
- Remote Processing Workstation with opensource Cryo-EM Processing Software Suites
- University of California users are charged $2060 a day for the G3 Krios microscope; $1840 for the G2 Krios microscope; University of California users are charged $1585 a day for the Talos Arctica microscope
- Lawrence Berkeley users are charged an additional 9.9% per UC Berkeley’s recharge policy
- External Non UC/Non academic user charge rates are $4250 a day for the G3 Krios microscope; $3750 for the G2 Krios microscope; $3500 a day for the Talos Arctica microscope
- Workstation usage is $44 a day for academic users and $370 for external Non UCB/Non academic user
- Screening Services for internal users at $220 per hour and $405 for external Non UCB/Non academic user
- Clipping Services for internal users at $220 per unit and $405 for external Non UCB/Non academic user
Users of the facility should use the RRID citation in their manuscripts: University of California at Berkeley Cal-Cryo QB3-Berkeley Core Facility, RRID:SCR_028363
Please ensure that grants are included in citations only when they are directly relevant to the facility. If you use a federal grant number for citation in any publication, please add the RRID when citing the facility. This will help maintain specificity and clarity.
Contact
| Kedar Sharma Scientific Director | kedarsharma14@berkeley.edu |
| Paul Tobias Computer System Analyst | ptobias@berkeley.edu |
| Associate Professor Stephen Brohawn Faculty Co-Director | brohawn@berkeley.edu |
| Professor James Hurley Faculty Co-Director | jimhurley@berkeley.edu |