Cal-Cryo at QB3-Berkeley provides researchers with access to state-of-the-art Cryo Electron Microscopes and related tools for high resolution structural biology imaging experiments.
The Cal-Cryo facility, housed in the basement of Stanley Hall, is led by Professors James Hurley and Steve Brohawn and began operations in February 2018. Our facility is used to collect image data of biological or soft material samples before it is processed using single particle or tomographic reconstruction methods Our staff offer consultation and training in specimen preparation, microscope operation, image acquisition, and data analysis.
The facility’s instrumentation features automated data collection. Both single-particle micrographs and tomography tilt series can be collected.
The current schedule can be viewed on this calendar.
Procedures and documentation for use of Cal-Cryo@QB3-Berkeley’s equipment are accessible in this folder.
Cal-Cryo@QB3-Berkeley instruments include:
- Krios G2 300kV CryoTEM
GATAN K3 Direct Electron Detector
Volta Phase Plate, BIO Quantum Energy Filter
- Krios G3i 300kV CryoTEM
GATAN K3 Direct Electron Detector
BIO Quantum Energy Filter
- Talos Arctica 200kV CryoTEM
Gatan K3 Direct Electron Detector
- FEI Mark IV Vitrobot
- Remote Processing Workstation with opensource Cryo-EM Processing Software Suites
- University of California users are charged $1872 a day for the G3 Krios microscope; $1672 for the G2 Krios microscope; University of California users are charged $1440 a day for the Talos Arctica microscope
- Lawrence Berkeley users are charged an additional 9.9% per UC Berkeley’s recharge policy
- UC Berkeley affiliated startup rates are $4000 per day on the Krios and $3000 per day on the Arctica
- Non-academic users can email the Facility Manager to inquire about pricing – dtoso@berkeley.edu.
- Workstation usage is $40 a day for academic users
- Screening Services for internal users at $200 per hour
- Clipping Services for internal users at $200 per unit
Contact
| Dan Toso Facility Manager | dtoso@berkeley.edu |
| Paul Tobias Computer System Analyst | ptobias@berkeley.edu |
| Associate Professor Stephen Brohawn Facility Co-Director | brohawn@berkeley.edu |
| Professor James Hurley Facility Co-Director | jimhurley@berkeley.edu |