Cal-Cryo @QB3-Berkeley

The Cal-Cryo facility, housed in the basement of Stanley Hall, is led by Professors James Hurley and Steve Brohawn and began operations in February 2018. Our facility is used to collect image data of biological or soft material samples before it is processed using single particle or tomographic reconstruction methods. Our staff offer consultation and training in specimen preparation, microscope operation, image acquisition, and data analysis. 

The facility’s instrumentation features automated data collection. Both single-particle micrographs and tomography tilt series can be collected.

The current schedule can be viewed on this calendar.

Procedures and documentation for use of Cal-Cryo @QB3-Berkeley’s equipment are accessible in this folder.

Cal-Cryo@QB3-Berkeley instruments include: 

• Krios G2 300kV CryoTEM
  GATAN K3 Direct Electron Detector
  Volta Phase Plate, BIO Quantum Energy Filter
  
•  Krios G3i 300kV CryoTEM
  GATAN K3 Direct Electron Detector
  BIO Quantum Energy Filter
  
• Talos Arctica 200kV CryoTEM
  Gatan K3 Direct Electron Detector
  
• FEI Mark IV Vitrobot
• Remote Processing Workstation with opensource Cryo-EM Processing Software Suites
  

• University of California users are charged $2060 a day for the G3 Krios microscope; $1840 for the G2 Krios microscope; University of California users are charged $1585 a day for the Talos Arctica microscope
• Lawrence Berkeley users are charged an additional 9.9% per UC Berkeley’s recharge policy
• External Non UC/Non academic user charge rates are $4250 a day for the G3 Krios microscope; $3750 for the G2 Krios microscope; $3500 a day for the Talos Arctica microscope
• Workstation usage is $44 a day for academic users and $370 for external Non UCB/Non academic user
• Screening Services for internal users at $220 per hour and $405 for external Non UCB/Non academic user
• Clipping Services for internal users at $220 per unit and $405 for external Non UCB/Non academic user