The QB3 Cell and Tissue Facility (CTAF), located in Stanley Hall, provides a full service cell culture room with equipment and instrumentation for researchers to grow and assess mammalian cells to further their research. Instruments include flow cytometry, Luminex Xmap multiplex immunoassay system, qPCR, hypoxia chambers, micromanipulator, protein quantification, and liquid handling automation. There are microscopes for fixed and live cell automated epifluorescence, confocal, and multiphoton imaging. Tissue related instrumentation includes cryostats, sliding microtome, paraffin-embedding equipment, and histology processing and staining setup.

The High-Throughput Screening Facility (HTSF), located in the Li Ka Shing Center for Biomedical and Health Sciences, this facility is unique in providing researchers hands-on access to liquid handling automation in a core facility environment. Expert staff provide training on use of the cell culturing space, automated liquid handling, automated plate reader and high-throughput, high-content Opera Phenix confocal microscope with robotics for any size multi-conditional screening and validation experiments. The microscope provides high quality single or Z-stack images of 2D/3D based multi-well plate and slides with followup software tools for complex image analysis and bioinformatics assessment. We assist with some siRNA and arrayed CRISPR screening reagents, CRISPR cell line identification, along with other high-throughput fluidics projects. We also provide a lentivirus packaging service, and an amplification service, providing maxipreps of DNA and specialized CRISPR libraries with full validation, upon request.

Rates – CTAF

General citations

QB3 CTAF or HTSF acknowledgement: We thank [staff names here] of the [Cell and Tissue Analysis Facility (CTAF) or High-Throughput Screening Facility (HTSF)] at UC Berkeley. This work was performed in part in the [QB3 CTAF or HTSF], that provided the [name specific instrument used].

NIH citations for use of the Perkin Elmer Opera Phenix at the HTSF

Each publication, press release, or other document about research supported by an NIH award must include an acknowledgment of NIH award support and a disclaimer such as “Research reported in this publication was supported by the Office of the Director, National Institutes of Health, under Award Number S10OD021828. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.”

Prior to issuing a press release concerning the outcome of this research, please notify the NIH awarding IC in advance to allow for coordination.

The CIRM/QB3 Shared Stem Cell Facility and the High-Throughput Screening Facility are directed by Professor David Schaffer, Departments of Chemical and Biomolecular Biology, Bioengineering, and Neuroscience, and Director of the Berkeley Stem Cell Center. Professor Michael Rape, Department of Molecular and Cell Biology, serves as a scientific adviser for the HTSF. Both facilities are managed by Dr. Mary West.

The CTAF is located in UC Berkeley’s Stanley Hall in the northeast area of the campus.
The HTSF is located primarily in UC Berkeley’s Li Ka Shing Center for Biomedical and Health Sciences in the northwest area of campus.

Visit QB3-Berkeley’s core research facilities to learn more about these facilities that serve the Bay Area scientific community.

Learn about other campus research facilities that may have some complementary capability to the CTAF for your research:

• CRL Flow Cytometry Facility and Molecular Imaging Center
• MCB Cell Culture Facility
• CNR Berkeley Imaging Facility
• Berkeley Stem Cell Center
• UC Berkeley Free Statistical Consulting