Below is a list of CTAF equipment and instruments. For any resource that is not hyperlinked below, please email Mary West or the QB3-Berkeley admin team to request access.
Human Cell Culturing Room and Equipment
- Evos Calibration Measurements:
| Objective | Microns/Pixel |
| 2x | 4.309 |
| 4x | 2.155 |
| 10x | 0.862 |
| 20x | 0.431 |
| 40x | 0.215 |
Printed out at 70% (properties –> effects) for size nice for taping to side of Evos
- EVOS xl core Quick Start Guide
- Evos xl core User Guide
- gentleMACS Dissociator User Manual
- Scepter Pro Software Guide 00110230TP2 RevE
- Scepter User Guide RevE
- Zeiss AxioObserver A1 detailed description
- Zeiss AxioCam MRm Info 48-0065 e
Specialized Imaging Equipment
ImageXpress Micro by Molecular Devices; Automated Epifluorescence Microscopy and Analysis Workstation
- MetaXpress Software Analysis Guide
- IXM objective options
- IXM filter sets
Lionheart FX by Agilent/Biotek; Automated Inverted Epifluorescence Microscopy and Analysis Workstation for Microfluidic Devices, and Slide imaging
- Lionheart Cell Imaging Training Guide 1321031 Rev J
Multiphoton/Confocal (Swept Field Confocal) Microscopy by Prairie Technologies
- Ultima Manual v2.5i
- Prairie 2P SFC Point Calibration 07032012
- PrairieView Manual 120313
- LaserCheck Operating Instructions RevAC
Perfusion and Heated Stage Platform by Warner Instruments/Harvard Apparatus Company
BioPlex MAGPIX Multiplex Reader by BioRad
BioPlex and pierceable seals:
- The probe is capable of piercing through sealants, below is a list of sealants could use. Piercing the following materials, at minimum:
- Corning Costar Aluminum Sealing Tape (Product #6570)
- Excel Scientific AlumaSeal Films for Cold Storage (#FC100)Please note that the probe could get clogged and maintenance procedures would need to added to the protocol (for instance add wash/rinse command after every 4-5 wells or as needed).
Genomics Equipment
BioRad qPCR CFX Connect 96w
BioRad qPCR assay design optimization
BioRad Gene_Pulser with mammalian cell and bacterial/yeast shock pods
Gene Pulser XCell Manual 4006217A
Electroporation Quick Guide 4006218A.pdf
BioRad Thermal Cycler T100
T100 Instruction Manual 10020523.pdf
Histology Equipment
Cryotome Thermo “Red” 77210163 620 & E Operator Guide (Issue 2)
Microtome 386040 HM 500 OM Instruction Manual
Rotary Microtome HM 355 S Instruction Manual