Stem Cell Culture Room

EVOS xl core is a light weight microscope with a video screen and high clearance to make it optimal for having it fully in one of our hoods for isolating iPS colonies and documenting as you go along. Objectives are 4x, 10x, and 20x, Plan LWD Phase, and the fixed stage has large area to accommodate plates of any size. Images are saved to your own flash drive from color camera acquisitions (1/2-inch CCD, 2048 x 1536, 3.1 Megapixels, COLOR camera); images are 24 bits/pixel, full color TIFF, JPG or BMP, 2048 x 1536 pixels.

The Scepter 2.0 handheld automated cell counter provides a fast and convenient method for counting cells and particles and is compatible with both the 40 and 60uM sensor. The system employs the Coulter principle in a miniaturized, handheld, format enabling rapid cell counting; what used to take 10 minutes now takes less than 30 seconds. The user prepares a dilution of the cell culture of interest and uses the Scepter cell counter to aspirate a sample of this dilution into the Scepter sensor. The Scepter cell counter’s screen displays:

• Cell concentration
• Average cell size
• Average cell volume
• Histogram of size or volume distribution

The Scepter cytometer counts cells or particles between 8 and 25 µm.

• Samples should be diluted within the Scepter operating range (10,000-500,000 cells/mL).
• A sample size of 100 µl or more is recommended.

The gentleMACS™ Dissociator is a benchtop instrument for the automated dissociation of tissues into single-cell suspensions or thorough homogenates. A single sample or two samples in parallel can be processed. Two types of unique gentleMACS Tubes used with the instrument enable the time-saving and easy dissociation or homogenization of tissues in a closed system. The instrument offers optimized gentleMACS Programs for a variety of specific applications. Special protocols have been developed for various tissues. The standardized tissue dissociation or homogenization procedures make for reliable and reproducible results.