In this section, we will cover the core techniques in protein expression, purification, and analysis.

Being able to purify and experiment with proteins is essential for understanding their unique properties and roles in biological systems. Here, we will induce expression of the His6-GFP protein in E. coli, isolate and purify this protein, determine the quantity and quality of our purified protein, and finally, use the purified GFP protein in a functional assay.

As we discussed in the Experimental Overview page, the protocols in this section include:

Protein Expression, Purification, and Analysis Protocols